FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18779770
Sequences flagged as poor quality0
Sequence length41
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG19250.013.7257185
ATACTGT23800.011.9105736
TCTATAC17550.011.6626123
GTATACC10550.011.2778321
TCGTGTA12300.010.38641313
GTATAGA17700.010.379711
TCCTATA15300.010.2903472
TACACTG30100.010.0569995
TAGACAG28700.09.9988685
GTCCTAA26800.09.8585111
TAGACCG11800.09.7870275
GGTATAC9900.09.7188433
GTCTAAG17300.09.6083021
CTACACT43400.09.5547084
CTATACA17300.09.5069354
CCTATAC26100.09.4507373
CTAGTAC9650.09.4267743
TACACCG16400.09.3891795
TAGGACC23900.09.3706754
AGGACCG33250.09.2621085
Produced by FastQC (version 0.11.5)