FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78626414
Sequences flagged as poor quality0
Sequence length41
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG112750.012.2797175
GTATAGA84100.010.9920611
TAGACCG42850.010.7840415
ACCCGTT66250.010.40700733
ATACTGT144500.09.8722976
GTATAGG98400.09.8216661
TCTATAC87000.09.818333
TACACTG136950.09.63690955
TAGACAG151450.09.3152445
TAGGACC98700.09.1331074
GTGTAGG170050.09.0809891
GTATTAG123900.08.8318141
GTATATA201950.08.8082791
TACACCG63200.08.8072145
CCTATAC99650.08.80033
GACCCGT79950.08.77690532
GTACTAG48150.08.7631851
CTAGCCT169200.08.6794084
TAGACTG118150.08.6370085
ACGAGAC82500.08.63295128
Produced by FastQC (version 0.11.5)