FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40879184
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGA780540.19093825356200847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG437080.1069199424332932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA90700.029.77503827
CGACGAG92300.029.27773728
CGAGTGT95550.028.56729731
GACGAGT95650.028.50154329
TGCCGAC95700.028.30791325
GCCGACG100200.027.07141726
TGACGCT104150.026.37346619
ACGAGTG103100.026.2939830
CTGCCGA103800.026.19705424
TCTGACG107750.025.44682517
ACGCTGC113100.024.2262121
GACGCTG133600.020.83944920
CTGACGC133900.020.5267118
CGCTGCC140000.019.84951422
GCTGCCG143350.019.44404623
ATCTGAC145550.019.15425116
GAGTGTA160000.017.22951132
AGTGTAG166200.016.83096333
CATCTGA168450.016.61229315
GTGTAGA170150.016.3204434
Produced by FastQC (version 0.11.5)