FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18080459
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA218680.12094825689989398TruSeq Adapter, Index 5 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29700.027.788582
AGATCGG32300.026.9740121
ACACGTC27000.026.60534714
ATCGGAA30700.026.2853743
CGTCTGA28050.025.31573917
TCGGAAG31850.025.1935774
CGGAAGA33800.023.7853495
ACGTCTG31450.022.73606316
CACGTCT32250.022.47859415
GCACACG33500.021.39393412
CACACGT34350.021.00842913
AGAGCAC52500.015.6606129
GAAGAGC55850.014.7757787
GTCACAC56350.014.32300530
CCAGTCA55900.014.27454627
CAGTCAC55900.014.247251528
GAGCACA59000.014.14173810
TCTGAAC59050.013.69692519
AGCACAC55650.013.35246511
GTCTGAA65050.012.42771818
Produced by FastQC (version 0.11.5)