FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37773924
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT32150.026.24193645
TCTCGTA36350.022.40524544
TATCTCG40500.019.99824542
ATCTCGT48400.017.0129743
CGTCTGA57700.014.85457417
ACGTCTG69000.012.48700916
ACACGTC72900.011.63382714
CACGTCT78050.011.35617415
CACTATC85550.09.940536539
ACTGACC92450.09.70998333
GACCACT92750.09.55728936
CCACTAT97550.08.97170138
ACCACTA103400.08.87752837
CACACGT102450.08.60761513
ACTATCT104450.08.31412240
TGACCAC114050.07.96963635
GCACACG116350.07.637293312
CAGTCAC122400.07.609877628
CACTGAC123700.07.602545332
CTATCTC118350.07.52785741
Produced by FastQC (version 0.11.5)