FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35818548
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT38250.024.75421545
TCTCGTA43350.021.32320244
ACGGCTA48500.019.66168833
AGTCACG55400.017.61849429
ATCTCGT60350.015.46577743
GTCACGG69000.014.34160830
CGGCTAC68100.014.1349234
CACGGCT75050.013.36536632
TCACGGC79450.012.42692531
GGCTACA97200.010.36593435
GCTACAG107550.09.7029236
CGTCTGA101900.09.68896417
AGATCTC105150.09.45397741
CAGTCAC116750.09.03448928
CCAGTCA118750.08.92021827
CAGATCT119950.08.5687540
ACGTCTG118000.08.46214916
GATCTCG120200.08.36381842
CTACAGA128650.08.04159737
TCCAGTC136300.07.821140326
Produced by FastQC (version 0.11.5)