FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27303508
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT652030.2388081414300316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT31650.030.49119834
CTCGTAT32050.030.25053245
ACCGATG33650.028.94634633
CACCGAT34950.028.06280132
TATCTCG33350.027.45277842
TCTCGTA33800.026.9539344
CGATGTT38350.025.39881335
ATCTCGT38750.023.74320643
TCACCGA43850.022.62354931
GTCACCG45550.021.6804330
CGTCTGA53350.018.34142317
ACGTCTG63400.015.5404316
ACACGTC63350.015.33964414
CACGTCT70750.013.95777815
GTTTATC80050.011.858726539
CACACGT86350.011.41015413
AGTCACC94450.010.836802529
GCACACG97950.010.19666912
CCAGTCA103650.010.135159527
CAGTCAC104650.09.95234828
Produced by FastQC (version 0.11.5)