FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39120319
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTATCTCGTAT621940.15898132119014674TruSeq Adapter, Index 4 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT91200.033.56245445
TCTCGTA93500.031.91931744
TATCTCG95750.031.19290242
ATCTCGT111600.026.72242443
GACCACT137950.022.644936
CACTATC133950.022.58268439
ACTGACC147450.021.55203633
CCACTAT143850.021.16907938
ACCACTA152850.020.62875437
ACTATCT150700.020.20700540
CTATCTC160150.018.97251741
CAGTCAC168850.018.92658828
CACTGAC170450.018.7625532
TGACCAC174200.018.19088435
AGTCACT176700.018.17494829
CCAGTCA176300.018.03754827
GTCACTG185750.017.301730
CGTCTGA191450.016.44591717
TCCAGTC197200.016.26271426
CTCCAGT198600.016.19336925
Produced by FastQC (version 0.11.5)