FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35715881
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA756150.07.01244169
TCTGAAC720300.06.623874769
CCAGTCA340000.06.585201769
CTCCAGT451050.06.47831569
ACGTCTG849600.06.46445469
CAGTCAC309800.06.45877169
CACGTCT943900.06.392447569
AACTCCA564150.06.372009869
TCCAGTC398000.06.275651569
GTCTGAA806750.06.127884469
CTGAACT706450.06.114011369
ACACGTC1008650.06.06759369
TGAACTC747600.05.88361569
GTCACAC214400.05.85706469
GAACTCC678000.05.79049869
AGTCACA342350.05.703604769
CACACGT1136650.05.526962369
GCACACG1225700.05.34495669
AGCACAC1420900.05.14968369
GAGCACA1534450.05.0091769
Produced by FastQC (version 0.11.5)