FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33765598
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA30300.024.02842744
TATCTCG34550.021.20136642
ATCTCGT42700.017.0503943
GGTATCT71350.011.09919840
TCCAGTC199350.010.437942544
GTATCTC77350.010.32335941
GTGGTAT79850.09.64255838
TGGTATC82400.09.58433239
AGTGGTA84950.09.45210637
AACTCCA403350.09.09607344
GTCACAC93600.08.74218430
CTCCAGT255000.08.60855744
GAACTCC524800.07.93827544
CCATAGG70700.07.342155511
GTTGAGC67350.07.1593425
CTGAACT689550.07.08472144
GCCATAG72150.07.012008710
TGAACTC617950.07.00861844
CAGTCAC123000.06.81350828
ACTCCAG456750.06.70830144
Produced by FastQC (version 0.11.5)