FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27367851
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10300.013.06386449
GTCACCG56400.09.97057569
CGTATGC14300.09.16814547
CTCGTAT15300.08.79396845
ACCGATG31150.08.74945169
CACCGAT36400.08.62485469
TCACCGA52650.08.45284869
TCTCGTA16450.08.17907344
CGTCTGA385100.07.767071269
CCGATGT28250.07.69367569
ACGTCTG442800.07.6899169
ACACGTC541100.07.49155669
TCGTATG18600.07.419215746
CACGTCT499750.06.88952869
TCTGAAC395650.06.8100769
GCACACG690500.06.76997969
ATGCCGT20950.06.752193550
CACACGT630850.06.62261969
TGCCGTC19350.06.597486551
TATCTCG19900.06.58773242
Produced by FastQC (version 0.11.5)