FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29693699
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA465350.08.12536769
ACGTCTG530250.07.36508769
ACACGTC651300.07.35755169
CACGTCT600350.07.309618569
TCTGAAC457950.07.058845569
CACACGT761600.07.030349769
GTCTGAA523400.06.84847769
GCACACG834300.06.624489369
CTGAACT449800.06.5424769
AACTCCA372400.06.299576869
CTCCAGT298450.05.90692569
CATCACG48050.05.887522769
CCAGTCA215750.05.884494369
AGCACAC993850.05.80400369
TCCAGTC270850.05.770075369
GAGCACA1085550.05.676017869
CAGTCAC197100.05.601127669
AGAGCAC1160450.05.586148769
TGAACTC503650.05.45935869
GAACTCC458900.05.43541169
Produced by FastQC (version 0.11.5)