FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50374927
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1634630.3244927779250181TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA190850.040.4091334
GATCGGA196700.040.3618851
ACCGATG193050.040.0625732
CCGATGT192050.039.9962933
TCTCGTA191050.039.83731541
CTCGTAT190150.039.82921242
CACCGAT193250.039.81622331
CGTATGC190150.039.56855844
TATCTCG197200.039.11919439
ATCGGAA205450.038.7513542
GTCACCG198850.038.72810429
TCGTATG196850.038.4734743
ACACGTC207550.038.22133313
CGTCTGA209600.037.8227616
ATCTCGT203850.037.5409140
TCGGAAG212600.037.4674573
TCACCGA206000.037.3411930
CGGAAGA219700.036.346774
ACGTCTG224700.035.418115
CACGTCT232450.034.2372414