FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32806364
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1028670.31355806452674856No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA930120.2835181612933393No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG813970.24811344530591686No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG524340.15982874542268688No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA401570.12240612827437994No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC400570.12210130936790191No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT372010.11339568139888956No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG364680.11116135881440564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG190800.019.833345
ATAGGGG211950.017.7631138
GTTGTCG31250.017.60357526
GGCATAG219450.017.24431235
GAGCTTA226200.016.67959644
GTCACTC292200.016.2995198
GGGAGGT304450.015.9736243
GGTCACT296750.015.957247
TGTCGTT34650.015.94357728
TGGCATA242450.015.65159234
AGGTCAC307250.015.6508716
CATAGGG244850.015.61589837
GGAGGTC313700.015.5272264
ATATAGC39200.015.5200866
AGGGATC193850.015.3374912
ACTCTCC317400.015.18088611
GAGGTCA322600.015.1413435
GCATAGG257450.014.8428736
GTCGTTC38600.014.72118729
TCACTCT334900.014.4108849
Produced by FastQC (version 0.11.5)