FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26562102
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340660.12825039223175938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA69250.011.93760545
TACGGTT30650.011.5372335
CATGCAT48500.010.9809749
TTACGGT32350.010.79149434
TGTTACG33400.010.78881832
CGGTTGG33650.010.30909537
GTGTCGA64650.010.09872627
ATAGGGG52400.09.97475638
GTAACGA65250.09.93121418
GGTAACG65700.09.86010417
GTTACGG36550.09.8590433
GGCATAG49650.09.844027535
ATCGGTT70100.09.8360286
TAATCGG68500.09.8335944
AATCGGT70300.09.7105915
CGTGGCG58550.09.6235111
TCAGACG38250.09.5077842
GCATTCG15900.09.4608253
GTCGAGG69900.09.40669629
ATAATCG76150.09.2283873
Produced by FastQC (version 0.11.5)