FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39776262
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA654770.16461325601686755No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG611820.15381535851709746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG118750.016.8699745
ATAGGGG135800.015.09690838
GGCATAG141200.014.57761235
CATAGGG152550.013.51090137
GAGCTTA151800.013.41943444
GTCACTC213200.013.3089148
GGGAGGT238250.012.9442963
GCATAGG164600.012.6704836
ACTCTCC232300.012.40888311
TGGCATA168750.012.34365334
AGGGATC136200.012.2960862
GGAGGTC248500.011.9395054
GGTCACT236800.011.9161987
GTTGTCG25250.011.4019826
TCACTCT258750.011.3400869
AGGTCAC262750.011.3217156
ATAGCTC55650.010.99983739
TAGGGGA253150.010.9258839
CACTCTC262900.010.92147710
ACCCGGC58950.010.845075
Produced by FastQC (version 0.11.5)