FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42189308
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT590860.14004970169219177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35600.027.4200926
TCGACGA40600.024.209437
CGGTATA46350.024.0759620
GACCGCG155150.023.2676475
AGACCGC158900.022.9733224
ACCGCGT161000.022.5619096
GTTCGAC43550.022.4145855
CGACGAG41650.022.2490448
CAGACCG170750.021.6687743
TCAGACC215100.018.8756522
CCGCGTT193650.018.6998257
CGCGTTC200950.017.9981218
CTCAGAC275650.016.9974611
AGTTCGA62900.016.0912954
ATGACGG65000.015.95234216
CAGTTCG67750.015.9021323
CCCGTAT25750.015.5562211
GCGTTCT236150.015.3915579
GTATAGA82350.015.08216622
ACGAGGA82600.014.97655110
Produced by FastQC (version 0.11.5)