FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42189308
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG90300.010.82582135
ATAGGGG95650.010.64523338
AGCTTAG100000.010.31902145
CATAGGG104400.09.42889837
GGGAGGT162500.09.2446453
TAATCGG60750.09.0934024
GAGCTTA112500.09.05233244
AGACTCA162600.08.9907934
GCATAGG109650.08.976912536
GCCGACA120600.08.948339
ATCGGTT64200.08.9196416
CCGTTAA38750.08.83592337
GTCACTC145000.08.7212228
GTAACGA59900.08.63876918
ATAATCG69250.08.6254023
ACCCGGC48300.08.6021295
AACTCTA100350.08.59912845
AGGGATC98400.08.5799042
CCGACAA125050.08.57594240
GGAGGTC155200.08.5513234
Produced by FastQC (version 0.11.5)