FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21644908
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG328110.1515876158956185No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA299790.1385037071998643No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG256100.11831882122113895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG104500.014.90249145
ATAGGGG107300.014.74776638
GGGAGGT136650.014.5310013
GGCATAG109100.014.42198635
GGAGGTC139450.013.8847844
AGGGATC74100.013.6861632
ACTCTCC139200.013.54143711
TGGCATA118400.013.32723134
CATAGGG118650.013.280148537
GAGCTTA119950.013.26460444
GCATAGG124300.012.73083436
TCAGACG48200.012.6895212
GTCACTC153400.012.56615458
GGTCACT151800.012.5044157
CGTGTAA10000.012.37746745
TGGGAGG141850.012.2063192
TATCCTA48600.011.9385895
CAGGGAT97150.011.6644941
CACTCTC168500.011.21324510
ACCGGTA12900.011.16501645
Produced by FastQC (version 0.11.5)