FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24981699
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG308480.12348239405174165No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT273710.10956420538090704No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT257200.10295536744718604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG8700.018.6227256
GTTCCTA49450.018.5650815
CCCGTAT13600.017.5424021
AGACCGC85500.016.8449924
GTCCGTT19200.015.82541
CAGACCG91900.015.7453523
TTCGGCG71750.015.7415844
ACGCTAC126750.015.44278424
AACGCTA127850.015.32751523
AGTTACC60300.015.2605434
TCACGCT131600.015.2499238
GCCGGCT113050.015.0711871
CCGGCTT138900.014.9723842
CGCTACC130800.014.89582325
CCTACTC63700.014.86955218
GACCGCG97500.014.8640555
TCGGCGC76400.014.75400845
CTACTCT65450.014.36881519
AAAACGC138600.014.36595221
TCGACGA11850.014.2395817
Produced by FastQC (version 0.11.5)