FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24981699
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG305900.12244963803302569No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG251450.10065368252175323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG91250.014.83527145
GGGAGGT134050.013.48477653
TCAGACG68950.013.1089012
GAGCTTA105100.012.709455544
GTCACTC135900.012.5894228
ATAGGGG105950.012.50304438
GGCATAG106400.012.49381735
GGTCACT140700.012.2398527
ACTCTCC146100.012.03366811
AGGTCAC145300.011.9142476
GTTGTCG16750.011.84837526
GAGGTCA152450.011.7980235
GGAGGTC151700.011.7230164
TGGCATA117300.011.46856234
TAGCGTA3856.184564E-1111.09984637
CATAGGG119350.011.04330937
TCACTCT164700.010.8521539
GCATAGG126600.010.48235636
CACTCTC165850.010.46511910
CAGACGT85300.010.3848863
Produced by FastQC (version 0.11.5)