FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46297780
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20300.019.0564696
ACGCTAC75700.015.89626524
AACGCTA75200.015.79214423
ACCGCGT47050.015.7270156
TCGACGA25200.015.6190167
CGCTACC79850.014.90132825
CCCGTAT30650.014.09751
AGACCGC53000.014.0467914
GTCCGTT31150.013.943461
CGGTATA37650.013.919209520
TTCGGCG50950.013.86956744
TCGGCGC51250.013.74237345
CGACGAG28050.013.4709048
CGCGTGT14700.013.0128651
GACCGCG57400.013.0092185
GTCGGTT42400.012.9507611
CCGCGTT58600.012.6657947
GTTCGAC30250.012.6400435
CGTACGC59700.012.5457015
CAGACCG63600.012.5190443
Produced by FastQC (version 0.11.5)