FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46297780
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC79050.011.6711619
CGCTCTC87150.010.68943311
CGGGACG46700.010.55154717
ATAGGGG74800.010.43661138
GGCATAG75300.010.33743435
GCGCTCT91700.010.20821910
CAGCGCT89850.010.1931058
GCAGCGC91150.010.0984247
CATAGGG80800.09.60596137
AGCTTAG80850.09.60005145
GTCAGGA175350.09.0741361
GCATAGG84050.08.99354536
ATAACGC49200.08.550837539
TAACGCC51300.08.33237640
GGACGTC104600.08.10965319
TCTCGGG65600.08.02655314
GACCTCC209700.07.86689425
TGGCATA102000.07.82981334
GAGCTTA102900.07.67406244
GTGCAAG156300.07.630255713
Produced by FastQC (version 0.11.5)