FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13774716
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG502830.365038379012678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391310.2840784521437683No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG176990.12848903745093546No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG160490.11651056907452757No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT153340.11131989944475079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19250.026.9791476
GTTCGAC20100.026.6211225
TCGACGA21000.025.1591267
CGACGAG21400.023.7435938
GCCGGCT113400.023.693471
CGGTATA32400.023.62950520
CCGGCTT136200.021.8056772
AGTTCGA24600.021.3857574
CAGTTCG26950.020.355183
TCACGCT157700.019.4746978
TTCACGC160000.019.2366967
ACGCTCA163000.018.91023310
ACGAGGA34200.018.8012610
ATGACGG38050.018.78975316
GTATAGA43300.018.62491422
CGGCTTC169150.018.5947173
TATAGAG45550.018.10450423
CGAGGAT31750.017.84467111
ATAGAGT46950.017.7213224
CACGCTC174500.017.6253499
Produced by FastQC (version 0.11.5)