FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43006081
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA697890.16227705100587986No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG624160.1451329638708535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG130800.014.835525545
GGCATAG144500.013.44962435
ATAGGGG145350.013.40711538
GTCACTC198850.012.2558228
CATAGGG159250.012.16446537
GAGCTTA164200.011.94107444
GCATAGG166000.011.65532336
TGGCATA169300.011.59698734
GGTCACT211550.011.5199747
ACTCTCC215600.011.51332411
GGGAGGT236150.011.4949123
GGAGGTC221450.011.4147544
GTTGTCG27350.011.2661226
AGGTCAC226400.011.1124046
GAGGTCA233800.010.9560165
TCAGACG96550.010.7234662
AGGGATC128800.010.2577432
AACTCTA100350.010.09481945
CGTGGCG176150.010.0558591
TCACTCT251600.09.8473149
Produced by FastQC (version 0.11.5)