FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45871831
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCAC651180.14195640021432762No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG637260.13892185816607147No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT580320.1265090115979892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC126000.024.3382594
CAGACCG133900.023.0870173
GACCGCG142650.021.4029755
CCCGGTT76200.020.861051
ACCGCGT154500.019.8196836
TCAGACC172450.018.6040062
TTCGGCG68650.017.5902644
GCAACGC152400.016.59624921
CGTACGC74150.016.463985
GTCCGTT31250.016.1884121
TCGGCGC74850.016.0131545
GTCGCTT70450.015.98926451
CGCCAAA81850.015.84018225
CCGTACG80950.015.2198164
ACGCCAA85800.015.11094324
ACGCTAC124150.015.09264424
AACGCCA154100.015.0604723
TACGCCA84400.014.8907467
CCGGTTC98700.014.8516982
CCGCGTT207150.014.8039147
Produced by FastQC (version 0.11.5)