FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45871831
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG589550.1285211396946418No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA582090.1268948693153321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG81900.013.80998138
GGCATAG88800.012.45806935
AGCTTAG90650.012.30977945
CATAGGG91800.012.17363737
AGGGATC142250.012.0634092
GAGCTTA98250.011.5399944
GCATAGG101100.011.07607236
ATAGCTC58200.010.93414339
GTCACTC144000.010.6951528
GCGTTCG18900.010.60181815
ATTTCTA362200.010.5263032
TGGCATA106800.010.46329834
GGTCACT150250.010.2055737
CAGGGAT190600.010.0571761
CATTTCT391250.09.9542831
GTTGTCG22300.09.88925926
CACCGAA384050.09.88242945
GGGATCA181050.09.7541253
GGGAGGT183350.09.58262353
ACTCTCC157950.09.57907211
Produced by FastQC (version 0.11.5)