FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47508264
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT6352701.3371778855148233No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2197460.4625426852052519No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG2066980.4350779897998378No Hit
GCCGGTTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1448070.3048038126587829No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1245810.2622301669452708No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1050870.22119730579926053No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA918600.19335583383977153No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG739950.15575184982553772No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG656670.13822226802477985No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG520250.10950726383098318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG1507000.021.2935453
AGACCGC1508000.021.2346324
TCAGACC1547600.020.8963132
GACCGCG1535550.020.8125765
CTCAGAC1577550.020.8071171
GCCGGTT372250.020.5349371
ACCGCGT1564900.020.4292666
CTACACG164200.020.3983245
CCGGTTT405950.020.0237562
CGACCGC18000.019.7697371
GTTTTCG750500.019.69489545
TTTCACG430250.019.540766
CGGTTTC430350.019.5260143
GAACGAT1649200.019.08170945
CGTGGTT779350.019.01435541
TCGTGGT785550.018.89665640
AACGCTA786600.018.87378123
CCGCGTT1697150.018.8465987
ACGCTAC787300.018.80552524
CGCTACC794700.018.54561625
Produced by FastQC (version 0.11.5)