FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36397362
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA906570.24907574345635267No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG799630.21969449324376858No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG539880.1483294311274537No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT414640.11392034400734867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG149600.017.35492945
ATAGGGG170300.015.20496738
GGCATAG169250.015.05978735
GTCACTC231850.014.3745378
GAGCTTA184950.014.09871444
CATAGGG184200.014.02072437
GGTCACT241450.013.7664477
TGGCATA188000.013.71314934
ACTCTCC252700.013.34839811
GGGAGGT265200.013.3153693
AGGTCAC260350.013.2169246
GCATAGG196100.013.13546636
AGGGATC162350.012.7953812
GGAGGTC272150.012.7434434
GAGGTCA279700.012.60038955
TCACTCT276550.012.3192369
CACTCTC282300.011.88499310
TATCCTA81550.011.8666785
TAGGGGA296800.011.30173339
ATAGCTC53950.011.21739839
Produced by FastQC (version 0.11.5)