FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48151579
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1120300.2326611137715754No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1030010.21390991144859445No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG587480.12200638321746417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163350.017.83433745
ATAGGGG180750.016.07276738
GGCATAG189850.015.27844735
GAGCTTA204100.014.44926444
CATAGGG206100.014.07400437
GTTGTCG29650.013.88921326
GTCACTC290950.013.7980648
TGGCATA212750.013.77081434
GCGTTCG36050.013.73958815
GGTCACT297250.013.4833517
GGGAGGT320750.013.4342343
GGAGGTC310550.013.2800774
ACTCTCC307950.013.10454811
AGGGATC203850.012.89170552
GAGGTCA323100.012.7917335
GCATAGG228900.012.74090136
CGTTCGC38900.012.73293316
CGCGTTC40050.012.536015514
AGGTCAC331050.012.5011186
TCACTCT332750.012.3624699
Produced by FastQC (version 0.11.5)