FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40969277
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG903900.22062874089772197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21200.022.0745166
AGACCGC74250.020.0610334
GACCGCG80000.018.646095
CGGTATA36250.018.5587920
CAGACCG88550.017.4316483
TCGACGA29200.016.8732387
TTCGGCG75600.016.42789844
ACCGCGT91650.016.3495376
CCGTACG77750.015.7141624
CGACGAG29700.015.6800678
GTTCGAC30400.015.5421095
TCGGCGC80050.015.31781245
CGTACGC80150.015.2707215
CGGCAGT324350.014.62919840
GTCGGTT33750.014.2160081
TACGCCA89300.014.0578827
TCCGTAC88050.014.0551743
AGTTGCA342800.013.90112344
GACGGCA340450.013.82489938
ACGGCAG344450.013.71009339
Produced by FastQC (version 0.11.5)