FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50281107
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT656700.13060571637772414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC138550.022.9983234
GACCGCG142050.022.4475155
ACCGCGT147850.021.5973196
CAGACCG154950.020.8253483
CCGCGTT169950.018.8020747
TTCGACG19550.018.5187636
CGCGTTC175200.018.2386388
TCAGACC202400.016.5874982
TTCGGCG66250.016.56511344
CGGTATA30650.015.9296820
GTCGGTT48800.015.2585991
CCGTACG72950.015.1660554
CGTACGC74600.014.9210585
CTCAGAC258050.014.8811381
GCGTTCT217400.014.8637489
GTCCGTT38050.014.8397141
CCCGTAT32200.014.60148051
TCGGCGC78350.014.15041945
TACGCCA82950.013.7714647
GTTCCTA67750.013.1769915
Produced by FastQC (version 0.11.5)