FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51043112
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA127700.019.98170523
ACGCTAC129000.019.72986424
CGCTACC134200.018.86490425
AAAACGC148700.017.35637321
TTCGACG25800.016.7422756
AAACGCT164750.015.7338622
AGACCGC80500.015.1481554
GAAAACG171950.015.14014620
GACCGCG84350.014.6692795
GCCGGCT174950.014.6642821
GTCGGTT50800.014.454561
CCGGCTT206350.014.3273242
TCGACGA32350.014.0477867
GCACTAC69650.013.957808525
CCCGTAT35200.013.9496981
TCACGCT194000.013.85757358
CCCGCGT19500.013.6300791
GTCCGTT40750.013.5974821
CAGACCG93500.013.5719593
TTCACGC204700.013.2873767
Produced by FastQC (version 0.11.5)