FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31524444
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA398330.12635591606310329No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG354000.11229381238254353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG105250.015.15177345
GGCATAG117000.013.3791935
ATAGGGG120850.013.36308938
GGGAGGT183250.012.56257253
TGCCCGT95000.012.44792644
GAGCTTA130300.012.41198744
GTCACTC167700.012.3669428
CATAGGG129300.012.26327237
GGAGGTC181300.012.0537434
TGGCATA131200.011.99808834
ACTCTCC171400.011.67311
GCATAGG135800.011.61006536
GGTCACT180850.011.31720357
TCAGACG62900.010.7832452
TATCCTA62750.010.6669045
AGGGATC91000.010.5138412
AAGCCGG163950.010.20774345
ATCCTAG71850.010.0977686
CGGCTAA38000.010.07363831
CACTCTC202300.09.97664410
Produced by FastQC (version 0.11.5)