FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36168589
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG516680.14285323654732565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19350.023.1235716
TCGACGA25150.018.3273357
CGACGAG26400.017.3744168
CGGTATA37050.017.01209620
GTTCGAC26150.016.9385935
AACGCTA108700.016.28655823
ACGCTAC108500.016.2389424
GTCCGTT30400.015.6828921
CGCTACC113550.015.49690725
CCCGTAT24200.015.3331871
GTCGGTT29300.015.1203721
AAAACGC122650.014.61186621
GACGGCA191950.014.35035338
TGACGGC194800.014.27905837
CGGCAGT197000.013.93717840
GTCCGAT22350.013.8856241
GACCGCG35050.013.728045
GAAAACG133150.013.47426820
AAACGCT134050.013.35363622
ACGGCAG207050.013.30416139
Produced by FastQC (version 0.11.5)