FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28004624
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG966820.34523584390920586No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT451220.16112339162275488No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG284740.10167606606680384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33250.031.0635096
CGGTATA56200.028.74368120
CGACGAG36200.027.9678948
TCGACGA37050.027.8119567
GACCGCG84650.026.6910535
AGACCGC84800.026.590814
ACCGCGT87050.025.9275596
CAGACCG93400.024.286973
GTTCGAC43150.023.7295515
CCGCGTT98350.022.9446187
ACGAGGA61950.022.88256310
GTATAGA75450.022.7221822
AACGACG20150.022.55512816
TTCGGCG41800.021.85311344
GACGAGG65250.021.2764159
TCAGACC114400.020.481512
CGAGGAT61650.020.47553311
TCGGCGC44650.020.45823545
CAGTTCG55650.020.421423
CGCGTTC110750.020.3757238
Produced by FastQC (version 0.11.5)