FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39417764
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA552070.14005614321502355No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG503170.12765056891608564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG100200.012.55360138
AGCTTAG102100.012.14918245
GTTGTCG28650.012.08944526
GGCATAG104050.011.99817735
AGGGATC145000.011.0522432
CATAGGG115400.010.9180437
GGTCACT160200.010.4786957
TGTCGTT33000.010.42768228
GCATAGG122000.010.34501936
GTCACTC161950.010.3237288
GGGAGGT176050.010.29235653
GGAGGTC177200.010.0100114
GAGCTTA125300.09.899528544
TGGCATA133100.09.58042934
CACCGAA378350.09.5380845
CAGGGAT187050.09.5302011
GTCGTTC37800.09.51991329
ACTCTCC179450.09.45568411
ATTTCTA372050.09.2077132
AGGTCAC189500.09.11996
Produced by FastQC (version 0.11.5)