FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23159103
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT455780.19680382266964314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC144650.018.9860174
GACCGCG145750.018.8272975
CAGACCG150650.018.528463
ACCGCGT149950.018.3748366
AACGCTA63050.016.99663223
ACGCTAC61650.016.83483324
TCAGACC170400.016.4997752
CCGCGTT168950.016.3350077
CGCGTTC171100.015.9194148
CGCTACC63550.015.51670725
AAAACGC72100.015.01761721
CTCAGAC202000.014.8289761
GCACTAC36700.014.23190225
AAACGCT78100.013.922626522
GAAAACG79400.013.88969420
GCGTTCT197000.013.8835419
GAACGAT197300.013.46479745
CTACACG18900.013.44911345
GTCCGCT26950.012.7862611
CGTTCTC222350.012.42207510
Produced by FastQC (version 0.11.5)