FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23159103
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG239280.10332006382112469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG12200.011.0576515
ATAGGGG35650.09.61153338
TGCCCGT86150.09.10979844
CCGCGTT14750.08.99334113
CGCGTTC14550.08.96255314
GCGTAGG19950.08.81290139
AGCTTAG42150.08.76735245
CGTTCGC14450.08.71400716
AGGGATC75750.08.6567392
TCTATAG196400.08.5308033
CGTAGGG20850.08.32291740
CGTAACG7051.8189894E-128.31406842
GGCGTAG21300.08.25512538
ATTTCTA168400.08.2280552
ATAGTGT203200.08.2153796
CTATAGT203500.08.1898544
GCCCGTG98450.08.14810245
GGCATAG42700.08.12618635
TATAGTG204050.08.1027315
TAGTGTG199700.08.0224637
Produced by FastQC (version 0.11.5)