FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25063639
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG263520.10514035890797821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG31200.016.64675519
AGCTTAG78800.016.30432745
ATAGGGG87150.014.99230938
GGCATAG85500.014.83194935
CGAACGC35900.014.60338920
GAACGCT45350.013.64429321
AACGCTG46500.013.50027222
CATAGGG97900.013.27572237
GCTTAAC41250.013.19186236
GTCACTC125000.013.0267248
GCATAGG102200.012.71720436
GGTCACT126950.012.5253097
TGGCATA103850.012.40604534
AGCGAAC43050.012.29099518
ACTCTCC133100.012.1955411
GAGCTTA108250.012.11696244
GGGAGGT139900.012.0429873
AGGTCAC143200.011.8903676
TTAACAC45650.011.87233338
GGCTTAA48600.011.47362535
Produced by FastQC (version 0.11.5)