FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46791779
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG756720.16172071594029372No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA680990.1455362490064761No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG627970.13420519873800907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG195700.015.89112645
ATAGGGG217550.014.35962338
GGCATAG222850.013.827970535
GGGAGGT309100.013.2811153
GAGCTTA240300.013.13881344
GGAGGTC310500.012.9314874
TGGCATA247150.012.61588334
GTCACTC314050.012.491628
CATAGGG255050.012.27559737
GGTCACT321200.012.1505477
ACTCTCC333100.011.84462611
GCATAGG264400.011.81628936
GTTGTCG26700.011.74194826
AGGTCAC343850.011.729386
AGGGATC177000.011.4828882
GAGGTCA359250.011.4268995
CACTCTC363700.010.78630910
TGGGAGG311950.010.6379352
TCACTCT378350.010.6122739
CACCCGG97350.010.3927991
Produced by FastQC (version 0.11.5)