FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50377594
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG2625880.5212396606316689No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1477200.2932255954899315No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1288890.2558458825961399No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG903200.17928605324025598No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC547200.10861971693209485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC238900.026.3446254
CAGACCG255100.024.8040583
GACCGCG254900.024.6027365
ACCGCGT274550.022.856296
TCAGACC298200.021.8678552
TTCGACG20850.021.7905546
CTCAGAC375650.018.751781
TTCGGCG65300.018.1931744
CGGCAGT708750.017.82560540
AGTTGCA725050.017.46831344
CAGTTGC752150.016.92732843
GTCGGTT43400.016.8993761
ACGGCAG763900.016.55377839
GTTGCAG776850.016.37659545
TCGGCGC73050.016.23000145
TGACGGC780500.016.1875437
CCGCGTT457250.016.1680151
TCGACGA30200.016.161417
GACGGCA782600.016.13791538
GCAGTTG800250.015.94925842
Produced by FastQC (version 0.11.5)