FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41618519
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31150.014.23509526
AGCTTAG113700.013.41457745
ATAGGGG116500.013.21902438
GGCATAG121250.012.58903635
TGTCGTT37700.011.68734928
CATAGGG132000.011.63246137
GTCACTC194000.011.1090568
GCATAGG139100.011.05467436
GAGCTTA144150.010.86092544
GGTCACT195200.010.7985157
TATAGCT51750.010.7051517
GGGAGGT211750.010.7037653
TGGCATA149350.010.44630834
ATATAGC50950.010.4313896
ACTCTCC210300.010.26363411
GTCGTTC45200.010.24540329
TCGTTCC45150.010.15718130
GGAGGTC221350.010.1174724
TTGTCGT48600.09.80662827
GCTAAGT48550.09.73943514
Produced by FastQC (version 0.11.5)