FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881705
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT437710.3683898901714863No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA144720.12180070116199652No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG127350.10718158715436885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC121100.021.5175234
CAGACCG125050.021.2157293
GACCGCG124150.020.9134085
ACCGCGT126300.020.521776
TCAGACC133600.019.874832
TACGCGA807.577252E-519.68524741
CTACACG18350.018.51263245
CCGCGTT141250.018.3164867
CTCAGAC160200.018.1270331
CGCGTTC142200.017.9881448
GAACGAT147300.016.66285345
GCGTTCT156800.016.2988119
CGTTCTC171700.014.87130910
AGAACGA172450.014.23275144
CCCGCGT6350.014.1913131
AATACGG175950.013.78579232
ATACGGA176400.013.76314833
TACGGAG177400.013.60866434
CAATACG178800.013.603806531
ACTCCCC185600.013.53119125
Produced by FastQC (version 0.11.5)