FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51840339
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1012970.1954018857785633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33600.024.5074396
TCGACGA42650.019.464117
AGACCGC89300.019.2246914
CGGTATA63950.018.82346220
GACCGCG95150.018.1832875
CGACGAG44800.018.0779158
CAGACCG101900.017.4225673
ACCGCGT100800.017.0525346
AACGCTA179950.016.75617223
ACGCTAC182800.016.45865224
CCGCGTT170800.016.4203851
CGCTACC188150.016.05068825
GTTCGAC51200.016.0390175
GTTCCTA105050.015.54739915
CCTACTC107150.015.4737618
CGGCAGT340400.015.20825740
AAAACGC204100.014.91589921
TGACGGC348550.014.84600737
GACGGCA351250.014.67424138
CGCGTTC192900.014.3943282
Produced by FastQC (version 0.11.5)