FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25031225
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT999720.39938916293549354No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG272440.10884005876660051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC205950.025.0600554
CAGACCG210600.024.5617223
GACCGCG212300.024.3418545
ACCGCGT216200.023.891686
TCAGACC230400.022.6960852
CCGCGTT249650.020.6815627
CTCAGAC273100.020.3183171
CGCGTTC260250.019.8219138
TTCGACG13550.018.5955586
GCGTTCT280650.018.4290779
CGGTATA19700.016.21717320
CGTTCTC323550.016.17327310
TTCGGCG66950.015.2911144
TCGGCGC66850.015.11204145
GTTCCTA39550.014.67592215
TCGACGA18450.014.6324527
CCTACTC40100.014.418817518
GTTCTCT378650.013.97434911
AACGCTA96800.013.83050523
CTACACG30300.013.81174445
Produced by FastQC (version 0.11.5)