FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25031225
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG542530.21674129012862936No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA432120.1726323821546888No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG367290.1467327308192068No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG323330.1291706658383679No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT306280.12235917339243284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163700.015.80537245
GGTCACT226950.015.6135177
GTCACTC227650.015.5354858
GGGAGGT233500.015.5135763
GGAGGTC242050.014.8539514
GGCATAG176750.014.76877135
AGGTCAC243650.014.70961956
ATAGGGG178300.014.65306338
GAGGTCA248350.014.6220965
ACTCTCC247100.014.34928711
TCACTCT252200.014.1744629
TGGCATA189850.013.84441434
GAGCTTA191750.013.66918444
CACTCTC266100.013.37491810
TGGGAGG214100.013.1255862
GTTGTCG14550.012.83476726
AGGGATC90850.012.7542572
CATAGGG215650.012.25084837
CTGGGAG253000.012.2103261
TCTCCCC303850.011.78080913
Produced by FastQC (version 0.11.5)