FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38008188
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA380620.10014158001954737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG87900.012.54161538
AGCTTAG94150.011.8478945
GGCATAG101600.011.06684835
CATAGGG109950.010.19047837
GTTGTCG23200.010.18937226
GTCACTC155500.09.9853688
GGTCACT156650.09.75369557
GCATAGG116000.09.67823636
AGGGATC105400.09.5664692
GGGAGGT173150.09.5073863
GAGCTTA121250.09.33781844
GGAGGTC173300.09.3315344
ACTCTCC179350.09.08714711
TGGCATA126450.08.95314234
ATTTCTA258000.08.8695262
AGGTCAC188950.08.537456
TCTATAG268400.08.3945655
GAGGTCA194300.08.38194755
TAGGGGA179200.08.36515539
TCACTCT195750.08.1397319
Produced by FastQC (version 0.11.5)