FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39532188
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1167150.2952404253465556No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG507570.12839410760669256No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG399630.10108977524846335No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGG396900.10039919874913071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG43300.021.4534426
CGGTATA54300.020.84800520
AGACCGC340900.020.6912964
CAGACCG344100.020.6165353
GACCGCG345850.020.3951535
ACCGCGT350200.020.1352126
TCGACGA49950.018.8224287
TCAGACC386050.018.5627822
CCGCGTT390700.018.0537517
GTTCGAC50150.017.985075
CGCGTTC402850.017.5036668
CTCAGAC432700.017.4320071
CTACACG85500.017.3117245
CGACGAG52300.017.2025628
GCGTTCT447750.015.818749
GTATAGA78850.015.44578922
CTCGGTT81650.015.3920741
ACGAGGA70700.015.33424810
CAGTTCG62450.015.30717853
CGTTCTC488250.014.69546110
Produced by FastQC (version 0.11.5)