FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39829200
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG464430.11660540507969028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19850.022.770066
TCGACGA25100.018.724047
CGACGAG24400.018.5239568
GTTCGAC25250.017.9004675
GACCGCG76650.016.8981825
CGGTATA38600.016.7288220
ACCGCGT78750.016.5331886
AGACCGC80450.016.1559334
GCACTAC67900.015.71253725
AACGCTA181750.014.91356523
GTCCGTT29050.014.8674841
ACGCTAC182550.014.83939224
CCGCGTT88400.014.7283237
CAGACCG91850.014.6648783
CGCTACC186150.014.57664325
CTACACG69750.014.02490145
TTCGGCG51800.014.02256844
CCCGTAT25000.013.9467841
CCGGCTT214700.013.9090292
CGCGTTC93350.013.8991758
Produced by FastQC (version 0.11.5)